Objective : In order to study the effect of water extract of Leonuri herba (ELS) on the subarachnoid hemorrhage animal model, the autologous whole blood was applied to the vicinity of the sprague dawley rat right femoral artery.
Methods : Following periarterial application of autologous whole blood (PAB) and intraperitoneal injection of ELS (10, 100 and 300 mghg, P.o.), the vasomotor responses to ELS were identified and the histological changes, neovascularized blood vessel were observed.
Results : Contractile response to ELS (10, 100 and 300 mg/kg, p.0.) was significantly decreased in the rat femoral arteries of PAB 5 and 7 days. In PAB 5 and 7 days, significantly increased vessel wall thickness and narrowed lumen were observed in comparison with the control group. In the arteries exposed to autologous whole blood for 5 and 7 days, a lot of newly generated capillaries in association with markedly curved blood vessel wall thickness, elastic lamina and irregularly oriented endothelial cells were observed in the periphery of the vessel wall. The number of neovascularized blood vessels were significantly decreased in the periphery of PAB with ELS (10, 100 and 300 mg/kg, p.0.) 5 and 7 days. In VEGF expression, Blood plus ELS (10, 100 and 300 mg/kg, p.0.)-exposed vessel were more weak expression than blood-exposed vessel to vein autologous whole blood for 5 and 7 days.
Conclusions : From these results, we concluded that ELS inhibited posthemorrhagic chronic vasospasm and neovascularization in rat femoral artery model.
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